Long oligos
New methods and applications have raised the demand for oligonucleotides with longer lengths than ever before. The success of applications like MLPA and Padlock (below) depends largely on the purity of long oligo's.
Cheap synthesis protocols with less reagent usage often fail to synthesize longer oligonucleotides with as little as possible n-x byproducts.
For this reason, Biolegio has developed special synthesis methods for the manufacturing of oligos up to 150 bases in length.
We strongly recommend that any oligonucleotide longer than 40-50 bases should be purified by poly acrylamide gel electrophoresis (PAGE) to separate the full-length material from the shorter failure sequences (random base deletions).
However, for best results, we recommend that researchers still design their sequences to be as short as possible.
Padlock probes are long oligonucleotides, whose ends are complementary to adjacent target sequences. Upon hybridisation to the target, the two ends are brought into contact, allowing Padlock probes circularisation by ligation. Padlock probes provide extremely specific target recognition, which is followed by universal amplification and micro array detection. Since target recognition is separated from downstream processing, Padlock probes enable the development of flexible and extendable diagnostic systems, targeting diverse organisms.