Amino modification
The amino modifier reagents are used to introduce a primary amino group into the oligo. This group can be used for conjugation with e.g. an activated NHS ester or isothiocyanate fluorescent label. Amino modifiers may also be useful to customers who wish to print their own oligonucleotide-based microarrays.
Amino Modifier C6 is used for standard 5' labeling. This modification has a primary amino group at the end of a six-carbon spacer. But also aminomodifier C12 can be used.
For 3' labeling aminomodifier C7 or C3 is used. This amino modifier contains a branched seven-carbon, or three-carbon spacer respectively.
Internal amino-functions can be introduced to oligonucleotide sequences by a number of products. Amino-Modifier C6-dA, Amino-Modifier C6-dC, Amino-Modifier C6-dG and both Amino-Modifier C6-dTcan be added in place of a dA, dC, dG and dT residue, respectively. Furthermore, also Amino Modifier C2 dT can be used for internal labeling.
Several aminomodifiers are available from Biolegio.
Amino modifier C6 (5')
Amino modifier C12 (5')
Amino modifier C6 dA (internal)
