Cy labeling
Cy® 3
- Excellent spectroscopic performance with high quantum yields, high extinction coefficient, and good stability of fluorescence signal against changes in pH, various buffer conditions.
- These oligos are tested by U.V. absorbance monitoring, and calculation of A260/A552 ratio.
Absorbance: 552 nm
Emission: 570 nm
ε = 150000 cm-1M-1
Cy® 3 (5')
Cy® 3.5
- Absorbance in the orange with high quantum yields, high extinction coefficient, and good stability of fluorescence signal against changes in pH, various buffer conditions.
- These oligos are tested by U.V. absorbance monitoring, and calculation of A260/A581 ratio.
Absorbance: 581 nm
Emission: 596 nm
ε = 150000 cm-1M-1
Cy® 3.5 (5’)
Cy® 5
- Absorbance in the red with high quantum yields, high extinction coefficient, and good stability in various buffers and conditions.
- These oligos are tested by U.V. absorbance monitoring, and calculation of A260/A643 ratio.
Absorbance: 643 nm
Emission: 667 nm
ε = 250000 cm-1M-1
Cy® 5 (5’)
Cy® 5.5
- Absorbance in the far red with high quantum yields, high extinction coefficient, and good stability in various buffer conditions.
- These oligos are tested by U.V. absorbance monitoring, and calculation of A260/A683 ratio.
Absorbance: 683 nm
Emission: 707 nm
ε = 250000 cm-1M-1
