Fluorescein
Fluorescein phosphoramidite is designed to produce the same fluorescein-type structure as had been previously prepared using fluorescein isothiocyanate (FITC).
Fluorescein-dT is inserted into the desired sequence as a replacement for a dT residue, either internally or at the 3’ end.
These oligos are tested by U.V. absorbance monitoring, and calculation of A260/A495 ratio.
Absorbance: 495 nm
Emission: 518 nm
ε = 68000 cm-1M-1
