HEX
HEX is the hexachloro derivative of Fluorescein.
The hexachlorofluorescein phosphoramidite is designed to take advantage of the multicolor detection capability of modern DNA sequencers and genetic analyzers, e.g. 3100 DNA analyzer.
These oligos are tested by U.V. absorbance monitoring and calculation of A260/A535 ratio.
Absorbance: 535 nm
Emission: 556 nm
ε = 73000 cm-1M-1
