LightCycler 640 Replacement dye
FRET (Fluorescence Resonance Energy Transfer) Probes
FRET probes are a pair of fluorescent probes placed in close proximity. The emission spectrum of one fluorophore overlaps significantly with the excitation spectrum of the other. During FRET, the donor fluorophore excited by a light source, transfers its energy to an acceptor fluorophore when positioned in the direct vicinity of the former. The acceptor fluorophore emits light of a longer wavelength, which is detected in specific channels. The light source cannot excite the acceptor dye.
Primary Conditions for FRET
- Donor and acceptor molecules must be in close proximity (typically 10–100 Å).
- The absorption spectrum of the acceptor must overlap the fluorescence emission spectrum of the donor
- Donor and acceptor transition dipole orientations must be approximately parallel.
Most of the fluorophore for the FRET probes techniques are available please contact Biolegio.
For example the LightCycler 640 Replacement dye available.