TET
TET is the tetrachloro derivative of Fluorescein. The tetrachlorofluorescein phosphoramidite is designed to take advantage of the multicolor detection capability of modern DNA sequencers and genetic analyzers, e.g. 3100 DNA analyzer.
These oligos are tested by U.V. absorbance monitoring and calculation of A260/A521 ratio.
Absorbance: 521 nm
Emission: 536 nm
ε = 73000 cm-1M-1
