Dr. Rogier Louwen " By making use of the synthetic guide RNA's provided by Biolegio, we now easily make a Cas9/gRNA (RNP) complex that together with or without a template (PCR product) can be transferred at high efficiencies into the eukaryotic cells by using lipofection methods. Compared to the plasmid based genome editing procedures we observed less toxicity, increased specificity and remove the introduction of foreign DNA (plasmid backbone) into the eukaryotic cells. This makes the genome editing procedure much cleaner and therefore our group encourage colleagues in the field to start using synthetic guide RNAs."

Biochemical methods are used for monitoring of drinking water quality. The use of high quality probes and primers is essential for producing reliable test results. In addition to this, in our opinion a good, transparent and proactive interaction between Vitens N.V. and the supplier is very important as well. This is why Vitens N.V., laboratory department microbiology, has chosen Biolegio B.V. as the supplier for probes and primers for our biochemical analyses