CRISPR RNA Synthesis
- sgRNAfor Cas 9 from S. pyogenes
- Modifications for nuclease resistance available
- Custom RNA up to 100 nucleotides in length
- Shortest turnaround times
- Best price
Many consider CRISPR RNA as a major breakthrough in the field of synthetic biology. The rate of publications emerging around CRISPR has reflected this and increased dramatically within a short period of time.
Biolegio is now official Partner from Synthego (California, USA) focusing on RNA Synthesis for CRISPR application only. Thus enables Synthego to offer synthetic RNA with highest quality, fastest turnaround time and best price in the market.
Your custom CRISPR RNA
Improve your CRISPR workflow and genome editing efficiency by using synthetic CRSIPRevolution RNA from Synthego. You order your custom CRISPRevolution RNA via Biolegio and we will be your service partner for e.g. ordering, shipping and customs from the US.
Download our whitepaper to learn more about CRISPR.
1.5, 3 or 10 nmol by OD260
your 17-20 nts target sequence
no annealing required
Te buffer Incl.
Nuclease free water Incl.
Ship in 5-7 business days
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Available with chemical modifications for increased exo-nuclease resistance.
Ship in 10-15 business days
Custom RNA 50
5 nmol by OD260
10-50 nts of your custom RNA sequence
Material for 25-50 transfections
Custom RNA 75
51-75 nts of your custom RNA sequence
Material for 25-50 transfections
Custom RNA 100
76-100 nts of your custom RNA sequence
Material for 10-20 transfections
Ship within 5-10 business days
Achieve up to 90% editing efficiency with high quality, high purity synthetic sgRNA
Receive your full-length sgRNA consisting of your 17-20 nts target sequence and optimized Cas9 S. pyrogenes scaffold sequence
No need to anneal crRNA & tracrRNA before RNP formation or transfection
100% DNA free: no risk of integrating DNA into the genome
A straightforward and cost-effective solution for your genome editing research
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Try chemically modified sgRNA for increased exo-nuclease resistance
Discontinued due to low request
Custom RNA 50
Custom RNA 75
Custom RNA 100
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Now available: chemically modified Custom RNA for increased exo-nuclease resistance
Receive all CRISPRevolution products of proven quality. Every CRISPRevolution RNA is
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Fully synthetic RNA (no DNA contamination)
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High-Quality Liquid Chromatography SPE purified
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Analyzed via Electrospray Ionization Mass Spectrometry (documents provided for each Oligo)
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Shipped in nuclease-free tubes
Mass-spec trace of synthetic AAVS1 gene 100nt sgRNA (Synthego). Large single peak represents full-length product.

Mass-spec trace of IVT-derived AAVS1 gene 105nt sgRNA. Large n-1, n+1 and n+2 species are present.
Whitepaper: Transforming Life Science: CRISPR-CAS9
Poster: Use of synthetic gRNA for improved CRISPR genome editing
Guide: How to perform Succesful CRISPR Experiments
Webinar: Achieve 90% Efficiency with CRISPR Editing
Electroporation of Cas9/Synthetic RNA Ribonucleoprotein (RNP) Complexes for CRISPR/Cas9 Genome Editing (Thermo Neon™ System)
Nucleofection (electroporation) of Cas9/ synthetic RNA ribonucleoprotein (RNP) omplexes for CRISPR/Cas9 genome editing (Lonza Nucleofection™ System)