CRISPR RNA Synthesis

  • sgRNAfor Cas 9 from S. pyogenes
  • crRNA & tracrRNACombi Kit for Cas 9 from S. pyogenes
  • Modifications for nuclease resistance available
  • Custom RNA up to 100 nucleotides in length
  • Shortest turnaround times
  • Best price

Many consider CRISPR as a major breakthrough in the field of synthetic biology. The rate of publications emerging around CRISPR has reflected this and increased dramatically within a short period of time.

Biolegio is now official Partner from Synthego (California, USA) focusing on RNA Synthesis for CRISPR application only. Thus enables Synthego to offer synthetic RNA with highest quality, fastest turnaround time and best price in the market.

Your custom CRISPR RNA 

Improve your CRISPR workflow and genome editing efficiency by using synthetic CRSIPRevolution RNA from Synthego. You order your custom CRISPRevolution RNA via Biolegio and we will be your service partner for e.g. ordering, shipping and customs from the US.

Download our whitepaper to learn more about CRISPR. 

3 nmol by OD260
your 17-20 nts target sequence
no annealing required
material for 10-20 transfections
Te buffer Incl.
Nuclease free water Incl.
Ship in 5-7 business days

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Available with chemical modifications for increased exo-nuclease resistance.
Ship in 10-15 business days 

5 nmol by OD260 each
your 17-20 nts target sequence & tracrRNA
annealing required
material for 10-20 transfections
Te buffer & annealing buffer Incl.
Nuclease free water Incl.
Ship in 5-7 business days

……..

Available with chemical modifications for increased exo-nuclease resistance
Ship in 10-15 business days 

Custom RNA 50

5 nmol by OD260
10-50 nts of your custom RNA sequence
Material for 25-50 transfections

Custom RNA 75

5 nmol by OD260
51-75 nts of your custom RNA sequence
Material for 25-50 transfections

Custom RNA 100

3 nmol by OD260
76-100 nts of your custom RNA sequence
Material for 10-20 transfections
……..

Now available: chemical modifications for increased exo-nuclease resistance
Ship within 5-10 business days

Achieve up to 90% editing efficiency with high quality, high purity synthetic sgRNA


Receive your full-length sgRNA consisting of your 17-20 nts target sequence and optimized Cas9 S. pyrogenes scaffold sequence 


No need to anneal crRNA & tracrRNA before RNP formation or transfection


100% DNA free: no risk of integrating DNA into the genome


A straightforward and cost-effective solution for your genome editing research  

 
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Try chemically modified sgRNA for increased exo-nuclease resistance

We offer 2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the first three 5’ and 3’ terminal RNA residues to achieve better in vivo stability & improved editing efficiency for difficult cell lines (e.g. primary cells, stem cells)

Receive your crRNA (17-20 nts target sequence with linker for annealing to tracrRNA) and optimized tracrRNA (Cas9 S. pyrogenes  scaffold sequence)

 

Annealing is required before RNP formation or transfection. Annealing buffer is included. 


100% DNA free: no risk of integrating DNA into the genome

 

A straightforward and cost-effective solution for your genome editing research

 

…..

 

Try chemically modified cr:tracrRNA for increased exo-nuclease resistance

We offer 2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the first three 5’ and 3’ terminal RNA residues to achieve better in vivo stability

 

Working with CPF1 or other Cas systems? Would you like to try a different scaffold sequence? Custom RNA brings all the flexibility you need for your CRISPR genome editing

Custom RNA 50

Custom RNA 50 is perfect for designing short guide RNAs for nucleases that don’t require long sequences, such as Cpf1. You can use it to design your own Cas9 crRNA and custom 3′ linker to a tracrRNA

Custom RNA 75

Custom RNA 75 is perfect for designing guide RNAs for nucleases that don’t require guides as long as S. pyogenes Cas9. You can use it to design your own Cas9 tracrRNA and custom 5′ linker to a crRNA

Custom RNA 100

Custom RNA 100 is perfect for designing full length sgRNA (100-mer) for CRISPR at a practical scale and price point. You can custom design your entire sgRNA sequence from 76-100 nucleotides

.......


Now available: chemically modified Custom RNA for increased exo-nuclease resistance

2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the first three 5’ and 3’ terminal RNA residues to achieve better in vivo stability & improved editing efficiency for difficult cell lines (e.g. primary cells, stem cells)


All three custom RNA catagories have a fixed yield and fixed price.
Synthego was founded by former SpaceX engineers and consists of a professional and multi-disciplinary team which includes former employees of organizations like SpaceX and NASA, as well as experienced veterans in the biotech, manufacturing and hi-tech industries - Ensuring highest professionality and quality for your CRISPR application.

Receive all CRISPRevolution products of proven quality. Every CRISPRevolution RNA is


  •          Fully synthetic RNA (no DNA contamination)
  •          High-Quality Liquid Chromatography SPE purified
  •          Analyzed via Electrospray Ionization Mass Spectrometry (documents provided for each Oligo)
  •          Shipped in nuclease-free tubes

 

 

Contrary to e.g. in vitro transcribed (IVT) RNA constructs, Synthego sgRNA is highly pure thus decreasing likelihood of “off target” effects and has positive effects on editing efficiency.

Mass-spec trace of synthetic AAVS1 gene 100nt sgRNA (Synthego). Large single peak represents full-length product.



Mass-spec trace of IVT-derived AAVS1 gene 105nt sgRNA. Large n-1, n+1 and n+2 species are present.

Whitepaper: Transforming Life Science: CRISPR-CAS9

Poster: Use of synthetic gRNA for improved CRISPR genome editing

Guide: How to perform Succesful CRISPR Experiments

Webinar:  Achieve 90% Efficiency with CRISPR Editing 

 

Lipofection of Cas9/synthetic RNA ribonucleoprotein (RNP) complexes for CRISPR/Cas9 genome editing (Thermo CRISPRMAX™ Kit)


Electroporation of Cas9/Synthetic RNA Ribonucleoprotein (RNP) Complexes for CRISPR/Cas9 Genome Editing (Thermo Neon™ System)

Nucleofection (electroporation) of Cas9/ synthetic RNA ribonucleoprotein (RNP) omplexes for CRISPR/Cas9 genome editing (Lonza Nucleofection™ System)