We love your DNAcr:tracrRNA

Optimized for SpCas9, the cr:tracrRNA Kit is designed for researchers conducting basic CRISPR genome editing experiments. The CRISPR kit contains all the necessary components to successfully anneal, transfect, target and edit a gene using CRISPR/Cas9 and synthetic guide RNA.


crRNA

You provide the 17-20nt RNA sequence that binds to the DNA 17-20nt target sequence that is opposite to the PAM sequence. An optimized 22mer Linker is added on the 3′ end of your target sequence. For example, you will receive a crRNA with this format:

5′-AAUUUCACAGCUGCACAUA+Synthego Linker-3′


tracrRNA

We have optimized the 72mer tracrRNA sequence based on S. pyogenes, and provide this with a proprietary linker to duplex with the crRNA. You do not need to provide a sequence for the tracrRNA.

 

Purity and Accuracy

With synthetic RNA you can control the exact amount of RNA for your transfection or microinjection. When forming ribonucleoprotein (RNP) complexes with Cas9 or Cpf1, you will get the cleanest possible edits. After transfection and genome editing have occurred, Cas9, Cpf1 and exogenous RNA will degrade, leaving everything in your cells clean and without continued expression of Cas9, Cpf1 or guide RNAs.


Chart CRISPRevolution Synthetic RNA Kit

This chart compares real, normalized data from an electrospray ionization mass spectrometer to show the superior product quality you can expect with a CRISPRevolution Synthetic RNA Kit.

CRISPR/Cas9 cleavage assay



Working with stem cells or other difficult targets?
Try Chemically Modified Synthetic RNA. 


Chemically modified sequences can provide additional RNA stability with improvements in editing efficiency for particular cell types (e.g. stem cells, K562, prokaryotic) and genomic targets that prove otherwise challenging to edit. For a chemically modified version of our synthetic RNA, we offer 2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the first three 5’ and 3’ terminal RNA residues. 



cr:tracrRNA KIT

  • Editing Efficiency ++
  • Good Reproducibility
  • General Cell Types and Basic Applications
  • 2-piece crRNA and tracrRNA
  • Your 17-20nt Target Sequence
  • Annealing Required
  • Material for 10 to 20 Transfections
  • Quantity: 5 nmol by OD260 each
  • Annealing Buffer; TE Buffer
  • Nuclease-free Water
  • Ships in 3-5 Business Days

 

 

cr:tracrRNA KIT (chemically modified)

  • Editing Efficiency +++
  • Good Reproducibility
  • Primary and Stem Cells; Therapeutic Applications
  • 2-piece crRNA and tracrRNA
  • Your 17-20nt Target Sequence
  • Annealing Required
  • Material for 10 to 20 Transfections
  • Quantity: 5 nmol by OD260 each
  • Annealing Buffer; TE Buffer
  • Nuclease-free Water
  • Ships in 7-10 Business Days 


Place your order

Optimized for S. pyogenes Cas9

 

Biolegio Application Note CRISPR

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 our whitepaper to learn more about CRISPR. 

 

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