Degenerated oligo synthesis - mixed bases
Standard "wobbles" or "mixed bases" are mixtures of two or more different bases at a given position within the sequence. Wobbles are often incorporated into oligonucleotide probes designed to hybridize to an unknown gene that encodes a known amino acid sequence. Since the genetic code is degenerate, the probe is prepared with wobbles at the degenerate sites.
The use of mixed base additions in the design of a degenerated oligo is optimal when the number of degenerate sites are small. The wobbles, except for the ones at the 3'-site are synthesized with no extra costs.
Use the standard code letters for equally mixed base ratios in your sequence to incorporate a wobble into your oligo.
Standard code letters:
|R = A/G||Y = C/T|
|M = A/C||K = G/T|
|S = C/G||W = A/T|
|B = C/G/T||D = A/G/T|
|H = A/C/T||V = A/C/G|
|N = A/C/G/T||I = Inosin|