Molecular Inversion Probe (MIP)

  • Ideal as target enrichment technique for NGS application
  • Extraordinary high specificity in multiplex reactions
  • High capture reproducability
  • Bias reduction: no fragmentation and PCR needed
  • Straight forward and easily automatable

What is a Molecular Inversion Probe?

A Molecular Inversion Probe is a single stranded oligonucleotide containing two annealing arms complimentary to the target of interest with a sequence gap in between. This sequence gap can target a SNP or a larger region of interest. In between the annealing arms of the MIP binding sites. Universal primers are included and other functionalities like index sequences or digestion sites can be incorporated depending on the experimental setup.

Multiple advantages

The advances in DNA analysis made a great leap forward with the emergence of Next Generation Sequencing (NGS). With these advances different target enrichment techniques have been developed to select the regions of interest for NGS analysis in a sensitive and cost-effective way. Amongst these techniques a solution phase “capture by circularization” method using “Molecular Inversion Probes” (MIPs) has gained increasing interest. Extensively used for research in Single Nucleotide Polymorphisms (SNPs) and Copy Number Variation (CNV), now the MIPs have shown multiple advantages as a Genomic partitioning technique allowing enrichment for regions of interest at a scale that is matched by Next Generation Sequencing platforms.

More about Molecular Inversion Probes

Biolegio Application Note MIPs Molecular Inversion Probe  

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