Dedicated and strictly monitored workflow for NGS oligo's
Highly optimized cross contamination elimination
- State of the art LC-MS QC included in NGS-SP & NGS-P Services.
Over the last decades NGS oligo technologies have evolved at a rapid pace, and concurrently so has the demand for high purity - high quality associated products like oligonucleotides. With the throughput of sequencing platforms increasing, multiplexing samples is now the common method for making sequencing increasingly economical. Even at very minimal amounts barcode cross contamination can be disastrous to a sequencing experiment. At Biolegio we dedicated ourselves to develop a workflow for NGS oligo’s which has proven itself moreover for various NGS applications.
Biolegio NGS oligonucleotides are synthesized in an environment which is monitored by externally executed qPCR assays using swaps of the facility, hardware and technicians. Thus enables the detection of contaminating oligos and high risk areas.
Synthesis and Purification is performed on media used only once for every single oligonucleotide to rule out the possibility of cross contamination, therefore HPLC purification is not available for our NGS oligos. Based on the high coupling efficiency we reach due to our optimized synthesis protocols of 99,5% and higher, the purity of our NGS-S grade oligo’s are fully compatible for most NGS applications.
At Biolegio we offer three NGS oligo services: NGS-S, NGS-SP and NGS-P. For all three categories we give the same attention to the synthesis workflow to exclude cross contamination on the highest level possible. You decide for the turnaround time, purity and quality control as well as documentation needs and importance of yields.
Within our NGS-SP & NGS-P service the purity and quality for every single NGS oligonucleotide is assessed using state of the art UPLC-MS and Capillary electrophoresis to ensure purity and correct mass.
Please find more information on our white paper.
Looking for a reliable and ultra-sensitive target enrichment strategy for your project? Please learn more about molecular inversion probes (MIPs).