ReadyMax™ assays for the BD-MAX™

  • Order custom or validated assays for the BD-MAX™ delivered in snap-in tubes
  • No more cumbersome pipetting of primers and probes
  • Reduce your batch-to-batch variation
  • Minimize handling errors associated with manual reaction set-up
  • Dried down, stable at room temperature

 

   

At PAMM laboratory of Medical Microbiology (Veldhoven , The Netherlands) a multiplex qPCR is developed for the detection of pneumonia causing bacteria. The qPCR has been validated for respiratory specimens, such as bronchoalveolar lavage (BAL) and sputum samples on the BD MAX™ system. The assay is manufactured and marketed by Biolegio.

Targets b-CAP assay

  • Legionella pneumophila
  • Mycoplasma pneumonia 
  • Chlamydophila pneumonia 
  • Chlamydophila psittaci 

Format

Primers and probes are dried in snap-in tubes (b-CAP) and ready for use on the BD MAX™. 

Available kits:

  • 24 rxn kit (BDT-14001-24)
  • 48 rxn kit (BDT-14001-48)
  • 96 rxn kit (BDT-14001-96) 

Protocol

Please download the protocol for usage of the b-cap  assay on the BD MAX™.

Ordering
Please contact Biolegio for orders and quotations.

At PAMM laboratory of Medical Microbiology (Veldhoven , The Netherlands) a qPCR is developed for the detection of Mycobacterium tuberculosis complex in respiratory specimens such as bronchoalveolar lavage (BAL) and sputum samples on the BD MAXTM system (Becton, Dickinson and Company, New Jersey, USA). The assay is manufactured and marketed by Biolegio.

Targets MTB assay

  • Mycobacterium tuberculosis complex
Format

Primers and probes are dried in snap-in tubes and ready for use on the BD MAX™. 

Available kits:

  • 24 rxn kit (BDT-14004-24)
  • 48 rxn kit (BDT-14004-48)
  • 96 rxn kit (BDT-14004-96)

Protocol
Please download the protocol for usage of the MTB  assay on the BD MAX™.

Ordering & pricing
Please contact Biolegio for orders and quotations.

At the Max von Pettenkofer-Institut for Medical Microbiology (Ludwig-Maximilians-University, Munich, Germany) two multiplex qPCR have been developed for the fast and reliable detection of neonatal pneumonia pathogens using the BD MAX™ system: NP-1 and NP-2 assay. Both qPCR assays have been validated for swabs (gynecological) and urine-samples, as well as respiratory specimens such as bronchoalveolar lavage and endotracheal aspirate. The assays are manufactured and marketed by Biolegio.

Targets NP-1 assay

  • Mycoplasma hominis
  • Ureaplasma parvum
  • Ureaplasma urealyticum

Format

Primers and probes are dried in snap-in tubes and ready for use on the BD MAX™. 

Available kits:

  • 24 rxn kit (BDT-14009-24)
  • 48 rxn kit (BDT-14009-48)
  • 96 rxn kit (BDT-14009-96)

Protocol
Please download the protocol for usage of the NP-1 assay on the BD MAX™.  

Ordering
Please contact Biolegio for orders and quotations.

At the Max von Pettenkofer-Institut for Medical Microbiology (Ludwig-Maximilians-University, Munich, Germany) two multiplex qPCR have been developed for the fast and reliable detection of neonatal pneumonia pathogens using the BD MAX™ system: NP-1 and NP-2 assay. Both qPCR assays have been validated for swabs (gynecological) and urine-samples, as well as respiratory specimens such as bronchoalveolar lavage and endotracheal aspirate. The assays are manufactured and marketed by Biolegio.

Targets NP-2 assay

  • Mycoplasma hominis
  • Ureaplasma parvum
  • Ureaplasma urealyticum
  • Chlamydia Trachomatis

Format

Primers and probes are dried in snap-in tubes and ready for use on the BD MAX™. 

Available kits:

  • 24 rxn kit (BDT-14010-24)
  • 48 rxn kit (BDT-14010-48)
  • 96 rxn kit (BDT-14010-96)

Protocol
Please download the protocol for usage of the NP-2 assay on the BD MAX™.

 

Ordering

Please contact Biolegio for orders and quotations.

At the Max von Pettenkofer-Institut for Medical Microbiology (Ludwig-Maximilians-University, Munich, Germany) two multiplex qPCRs have been developed for the detection of distinct atypical pneumonia causing pathogens by means of the BD MAX™ system: AP-1 assay and AP-2 assay. Both qPCR assays have been validated for respiratory specimens such as bronchoalveolar lavage, endotracheal aspirate and sputum sample.The assays are manufactured and marketed by Biolegio.

Targets AP-1 assay

  • Legionella pneumophila
  • Toxoplasma gondii
  • Pneumocystis jirovecii

Format

Primers and probes are dried in snap-in tubes and ready for use on the BD MAX™. 

Available kits:

  • 24 rxn kit (BDT-14007-24)
  • 48 rxn kit (BDT-14007-48)
  • 96 rxn kit (BDT-14007-96)

Protocol
Please download the protocol for usage of the AP-1 assay on the BD MAX™. 

Ordering
Please contact Biolegio for orders and quotations.

At the Max von Pettenkofer-Institut for Medical Microbiology (Ludwig-Maximilians-University, Munich, Germany) two multiplex qPCRs have been developed for the detection of distinct atypical pneumonia causing pathogens by means of the BD MAX™ system: AP-1 assay and AP-2 assay. Both qPCR assays have been validated for respiratory specimens such as bronchoalveolar lavage, endotracheal aspirate and sputum sample.The assays are manufactured and marketed by Biolegio.

Targets AP-2 assay

  • Mycoplasma pneumoniae
  • Chlamydia pneumoniae
  • PAN-Chlamydiaceae

Format

Primers and probes are dried in snap-in tubes and ready for use on the BD MAX™. 

Available kits:

  • 24 rxn kit (BDT-14008-24)
  • 48 rxn kit (BDT-14008-48)
  • 96 rxn kit (BDT-14008-96)

Protocol
Please download the protocol for usage of the AP-2 assay on the BD MAX™.  

Ordering
Please contact Biolegio for orders and quotations.

Introduction

E. coli (EHEC) typically cause acute bloody diarrhea, which may lead to hemolytic-uremic syndrome. There are > 100 serotypes of EHEC; O157:H7 is the best-known, but many others cause similar illness. EHEC have a bovine reservoir, so outbreaks often result from ingestion of undercooked beef, but many other foods (e.g., fresh produce, raw milk) and sources (e.g., direct exposure to animals) may be involved. Use stool tests to identify Shiga toxin, the Shiga toxin gene and cultures to identify EHEC.

Ludwig Maximilians University Munich

At the Max von Pettenkofer-Institut for Medical Microbiology (Ludwig-Maximilians-University, Munich, Germany) a multiplex qPCR has been developed for the fast and reliable detection of EHEC pathogens using the BD MAX™ system. 

The qPCR has been validated for bacterial culture. The assays are manufactured and marketed by Biolegio.

Targets EP-1 assay

  • EHEC stx1
  • EHEC stx2
  • EHEC eaeA
  • EHEC hlyA

Format

Primers and probes are dried in snap-in tubes and ready for use on the BD MAX™. 

Available kits:

  • 24 rxn kit (BDT-14014-24)
  • 48 rxn kit (BDT-14014-48)
  • 96 rxn kit (BDT-14014-96)

Protocol
Please download the protocol for usage of the EP-1 assay on the BD MAX™.  

Ordering
Please contact Biolegio for orders and quotations.

Introduction

Enteric pathogenic E. coli comprise a group of distinct E. coli pathotypes equipped with special virulence factors, which enables these E. coli strains to colonize the human gut and cause intestinal disease (diarrhoea). The respective pathotypes, enteroaggregativen (EAEC), enteropathogenic (EPEC), enteroinvasive (EIEC) and enterotoxigenic (ETEC) E. coli can be differentiated and detected by their distinct virulence gene repertoire. The specific virulence genes are detected with this multiplex PCR assay as described below.

Ludwig Maximilians University Munich

At the Max von Pettenkofer-Institut for Medical Microbiology (Ludwig-Maximilians-University, Munich, Germany) a multiplex qPCR has been developed for the fast and reliable detection of Enteric pathogenic E. coli  using the BD MAX™ system.

The EP-2  assay allows for the detection of:
  • EAEC - aggR
  • EPEC – eae and EAF
  • EIEC - ipaH
  • ETEC - LT

The qPCR has been validated for bacterial culture.

Format

Primers and probes are dried in snap-in tubes (EP-2) and ready for use on the BD MAX™.

The snap-in tubes are manufactured and marketed by Biolegio.

Available kits:
  • 24 rxn Kit (BDT-14016-24)
  • 48 rxn Kit (BDT-14016-48)
  • 96 rxn Kit (BDT-14016-96)
Protocol

Please download the protocol for usage of the EP-2  assay on the BD MAX™.

Ordering

Please contact Biolegio for orders.

Introduction

Bacterial meningitis as an acute infection and inflammation of the meninges affects both children and adults. The respective bacteria species causing bacterial meningitis vary according to the infected individual's age group. The MP-1 assay focuses on bacterial pathogens causing meningitis in newborns.

Ludwig Maximilians University Munich

At the Max von Pettenkofer-Institut for Medical Microbiology (Ludwig-Maximilians-University, Munich, Germany) a multiplex qPCR has been developed for the fast and reliable detection of bacterial  pathogens causing meningitis using the BD MAXTM system.

The qPCR has been validated for cerebrospinal fluid. The assays are manufactured and marketed by Biolegio.

Targets MP-1 assay

  • E.coli
  • Streptococcus agalactiae
  • Listeria monocytogenes

Format

Primers and probes are dried in snap-in tubes and ready for use on the BD MAX™. 

Available kits:

  • 24 rxn kit (BDT-14011-24)
  • 48 rxn kit (BDT-14011-48)
  • 96 rxn kit (BDT-14011-96)

Protocol
Please download the protocol for usage of the MP-1 assay on the BD MAX™.

 

Ordering

Please contact Biolegio for orders and quotations.

Introduction

Bacterial meningitis as an acute infection and inflammation of the meninges affects both children and adults. The respective bacteria species causing bacterial meningitis vary according to the infected individual's age group. The MP-2 assay focuses on bacterial pathogens causing meningitis in infant, adolescent and adults.

Ludwig Maximilians University Munich

At the Max von Pettenkofer-Institut for Medical Microbiology (Ludwig-Maximilians-University, Munich, Germany) a multiplex qPCR has been developed for the fast and reliable detection of bacterial  pathogens causing meningitis using the BD MAXTM system.

The qPCR has been validated for cerebrospinal fluid. The assays are manufactured and marketed by Biolegio.

Targets MP-2 assay

  • Neisseria meningitidis
  • Streptococcus pneumonia

Format

Primers and probes are dried in snap-in tubes and ready for use on the BD MAX™. 

Available kits:

  • 24 rxn kit (BDT-14012-24)
  • 48 rxn kit (BDT-14012-48)
  • 96 rxn kit (BDT-14012-96)

Protocol
Please download the protocol for usage of the MP-2 assay on the BD MAX™. 

Ordering
Please contact Biolegio for orders and quotations.

Introduction

The herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) and varicella-zoster virus (VZV) can cause a wide range of signs and symptoms, varying from triv­ial mucocutaneous lesions to life-threatening infections of the central nervous system, espe­cially in immunocompromised patients and newborns. Since antiviral drugs are available, rapid and sensitive laboratory diagnosis of these virus infec­tions is important.

PAMM

At PAMM laboratory of Medical Microbiology (Veldhoven , The Netherlands) a multiplex qPCR is developed for the detection of the herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2) and varicella-zoster virus (VZV). The assay is validated for swabs (E-swab) and CSF samples on the BD MAX™ system (Becton, Dickinson and Company, New Jersey, USA). 

Format

Primers and probes are dried in snap-in tubes and ready for use on the BD MAX™. 

Available kits:

  • 24 rxn kit (BDT-14005-24)
  • 48 rxn kit (BDT-14005-48)
  • 96 rxn kit (BDT-14005-96)

Protocol

Please download the protocol for usage of the HSV/VZV assay on the BD MAX™.

 

Ordering & pricing
Please contact Biolegio for orders and quotations.

At the Max von Pettenkofer- Institut for Medical Microbiology (Lud­wig-Maximilians-University, Munich, Germany) a multiplex qPCR has been developed for the detection of the ospA gene and p41 flagelin gene, both highly specific for B. burgdorferi sensu lato. The qPCR has been validated for cerebrospinal fluid, tissue sample and joint aspiration. The assay is manufactured and marketed by Biolegio.

Targets Bb assay

  • B. burgdorferi sensu stricto 
  • B. garinii 
  • B. afzelii
  • B. bavariensis 
  • B. spielmanii
  • B. valaisiana 

Format

Primers and probes are dried in snap-in tubes (Bb) and ready for use on the BD MAX™. 

Available kits:

  • 24 rxn kit (BDT-14015-24)
  • 48 rxn kit (BDT-14015-48)
  • 96 rxn kit (BDT-14015-96)

Protocol
Please download the protocol for usage of the Bb assay on the BD MAX™. 

 

Ordering

Please contact Biolegio for orders.

Introduction

Carbapenemases are β-lactamases with versatile hydrolytic capacities. They have the ability to hydrolyze penicillins, cephalosporins, monobactams, and carbapenems. Bacteria producing these β-lactamases may cause serious infections in which the carbapenemase activity renders many β-lactams ineffective. Carbapenemases are members of the molecular class A, B, and D β-lactamases. Class A and D enzymes have a serine-based hydrolytic mechanism, while class B enzymes are metallo-β-lactamases that contain zinc in the active site. The class A carbapenemase group includes members of the IMI, GES, and KPC families.The class D carbapenemases consist of OXA-type β-lactamases such as the OXA48 like carbapenemases,    The metallo-β-lactamases belong to the IMP, VIM, SPM, GIM, and SIM families and have been detected primarily in Pseudomonas aeruginosa; however, there are increasing numbers of reports worldwide of this group of β-lactamases in the Enterobacteriaceae

Ludwig Maximilians University Munich

At the Max von Pettenkofer-Institut for Medical Microbiology (Ludwig-Maximilians-University, Munich, Germany) a multiplex qPCR has been developed for the fast and reliable detection of Carbapenemases using the BD MAX™ system.

The Carba-1  assay allows for the detection of:
  •          KPC
  •          OXA48
  •          IMP 8-13 group (blaIMP-2,-8,-13,-15,-19,-20,-24,-33,-37)
  •          GES

The qPCR has been validated for bacterial culture.

Format

Primers and probes are dried in snap-in tubes (Carba-1) and ready for use on the BD MAX™.

The snap-in tubes are manufactured and marketed by Biolegio.

Available kits:
  •          24 rxn Kit (BDT-14017-24)
  •          48 rxn Kit (BDT-14017-48)
  •          96 rxn Kit (BDT-14017-96)
Protocol

Please download the protocol for usage of the “Carba-1”  assay on the BD MAX™.

 

Ordering

Please contact Biolegio for orders.

Introduction

Carbapenemases are β-lactamases with versatile hydrolytic capacities. They have the ability to hydrolyze penicillins, cephalosporins, monobactams, and carbapenems. Bacteria producing these β-lactamases may cause serious infections in which the carbapenemase activity renders many β-lactams ineffective. Carbapenemases are members of the molecular class A, B, and D β-lactamases. Class A and D enzymes have a serine-based hydrolytic mechanism, while class B enzymes are metallo-β-lactamases that contain zinc in the active site. The class A carbapenemase group includes members of the IMI, GES, and KPC families.The class D carbapenemases consist of OXA-type β-lactamases such as the OXA48 like carbapenemases,    The metallo-β-lactamases belong to the IMP, VIM, SPM, GIM, and SIM families and have been detected primarily in Pseudomonas aeruginosa; however, there are increasing numbers of reports worldwide of this group of β-lactamases in the Enterobacteriaceae

Ludwig Maximilians University Munich

At the Max von Pettenkofer-Institut for Medical Microbiology (Ludwig-Maximilians-University, Munich, Germany) a multiplex qPCR has been developed for the fast and reliable detection of Carbapenemases using the BD MAX™ system.

The Carba-2  assay allows for the detection of:
  •          NDM
  •          IMP 14-18 group (blaIMP-14,-18)
  •          VIM(blaVIM-1bis -34)
  •          GIM

The qPCR has been validated for bacterial culture.

Format

Primers and probes are dried in snap-in tubes (Carba-2) and ready for use on the BD MAX™.

The snap-in tubes are manufactured and marketed by Biolegio.

Available kits:
  •          24 rxn Kit (BDT-14018-24)
  •          48 rxn Kit (BDT-14018-48)
  •          96 rxn Kit (BDT-14018-96)
Protocol

Please download the protocol for usage of the “Carba-2”  assay on the BD MAX™. 

 

Ordering

Please contact Biolegio for orders.

Have your own in-house developed assay for the BD-MAX™ delivered in a ready to use snap-in tube. We synthesize the primers, probes and deliver the complete assay according to your specifications.

We also offer the option to commercialize your assay. In this case we produce, promote and sell your ReadyMax™ assay to our mutual benefit.

ReadyMax™ Assays are custom synthesized assays for use on BD-MAX™ platforms. The primer and probe mixture of your own Real-Time PCR assays is synthesized according to your protocol. The oligonucleotides are dried down and delivered in a sealed conical tube, which is compatible with the BD MAX™ cartridges. No more cumbersome pipetting of primers and probes. With ReadyMax™ assays you just snap in your assay and start your run, making it possible to increasing your laboratory throughput and efficiency. 

Besides custom assays we offer an ever growing portfolio of ReadyMax assays that have been developed and validated  by our partners. You will find them under the tab 'order validated assays'.

If you have developed an assay for the BD-MAX™, then Biolegio offers the possibility to market this assay. We produce, promote and sell your ReadyMax™ assay to our mutual benefit.