We love your DNASynthetic sgRNA

Achieve up to 90% editing efficiency with the highest quality synthetic sgRNA in the market. Biolegio is the first company to deliver a production scale, full length 100-mer synthetic sgRNA product at a practical price and volume.

Unlike custom developed solutions that are expensive and can take weeks before transfection readiness, CRISPRevolution sgRNA ships up to 4X faster and 80% lower cost.

Benefits of Synthetic sgRNA

  • No need to anneal crRNA:tracrRNA

    Higher efficiency and reduced lab time
  • Better in vivo stability

    When duplexed with Cas9 nuclease
  • Cost-effective

    Highly scalable for large numbers of experiments
  • 100% DNA-free

    No risk of integrating foreign DNA into cell line
  • A single oligonucleotide

    Ready for transfection out-of-the box
  • Optimized for SpCas9

    Optimized 80mer scaffold for use with SpCas9

No need to anneal, the sgRNA format arrives ready for transfection. It uses a single strand of user-defined 17-20nt RNA target sequence that will bind to the DNA 17-20nt target sequence that is opposite to the PAM sequence.
An optimized 80mer Synthego Scaffold is added on the 3′ end of your target sequence. For example, you will receive a sgRNA with the following format:

5′-AAUUUCACAGCUGCACAUA+Synthego Scaffold-3′


Synthego 100-mer sgRNA (MS Trace)

Synthego 100-mer sgRNA (MS Trace). Large single peak represents full-length product.

IVT-derived 105-mer guide RNA (MS Trace)

IVT-derived 105-mer guide RNA (MS Trace). Large n-1, n+1 and n+2 species are present.

CRISPRevolution sgRNA Purity vs. IVT 

CRISPRevolution sgRNA Efficiency & Consistency

Hi Quality In = High Quality Out

The chart below compares the editing efficiency (solid bars) and consistency (black lines) of sgRNAs against in vitro transcribed guide RNAs in HEK293T cells. The experiment was conducted by a third-party using three different gene targets and was replicated three times.

sgRNA against IVT

 

Working with stem cells or other difficult targets?
Try Chemically Modified Synthetic RNA.


Chemically modified sequences can provide additional RNA stability with improvements in editing efficiency for particular cell types (e.g. stem cells, K562, prokaryotic) and genomic targets that prove otherwise challenging to edit. For a chemically modified version of our synthetic RNA, we offer 2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the first three 5’ and 3’ terminal RNA residues.

sgRNA KIT

  • Editing Efficiency ++++
  • Excellent Reproducibility
  • General Cell Types and Basic Applications
  • Full Length 100-mer Single Guide
  • Your 17-20nt Target Sequence
  • No Annealing
  • Material for 10-20 Transfections
  • Quantity: 3 nmol by OD260
  • TE Buffer
  • Nuclease-free Water
  • Ships in 5-7 Business Days

 

sgRNA KIT (chemically modified)

  • Editing Efficiency +++++
  • Excellent Reproducibility
  • Primary and Stem Cells; Therapeutic Applications
  • Full Length 100-mer Single Guide
  • Your 17-20nt Target Sequence
  • No Annealing
  • Material for 10-20 Transfections
  • Quantity: 3 nmol by OD260
  • TE Buffer
  • Nuclease-free Water
  • Ships in 7-10 Business Days 


Place your order

Optimized for S. pyogenes Cas9

 

Biolegio Application Note CRISPR

Download
our whitepaper to learn more about CRISPR. 

 

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