DNA synthesis has been our core product for over 25 years. All custom oligos are manufactured under ISO 9001 and ISO 13485 quality standards. These quality standards reflect high and consistent quality as a result of stringent production and manufacturing procedures.
Custom DNA Oligos
Optimised synthesis for very short and very long oligos and everything in between
Your choice of plate, strip or tube, dried or dissolved at your desired concentration for optimal flexibility.
Standard DNA oligos are shipped within 24 hours.
Purified & modified oligos within 4 business days,
Select from virtually every oligo modification option commercially available
DNA oligos in tubes
Custom DNA oligos can be ordered in our webshop by entering your sequences and specifications (up to 200 bases) or by email. Login to our webshop and order your oligo's
DNA oligos in plates
For medium and high-throughput applications oligos can be ordered in plates. No additional charge for orders containing more than 48 oligos per plate. More information about DNA synthesis in plates.
Order your primes and/or probes delivered in any format according to your specifications. All fluorophore -quencher combinations possible, ideal for qPCR applications.
Oligonucleotides up to and beyond 200 bases are achieved with our B-pure Oligonucleotide Synthesis protocol. This protocol enables high quality long oligo synthesis at an affordable price. By using the B-pure protocol we can achieve a coupling efficiency >99,5%.
Oligonucleotides longer than 80 bases are synthesized automatically on this protocol. Additional quality control is performed on our state-of-the-art LC-MS system to guarantee highest quality and optimized functionality of the oligonucleotides.
Oligo prepaid service
With our oligo prepaid service we make oligo ordering easy. Credit can be easily transferred to your account. Oligos can then be ordered immediately, eliminating possible delays due to purchase authorization. Please contact Biolegio for more information.
Scale, yield & method
Oligo scale and length
Biolegio offers four different synthesis scales for DNA oligos: 10 nmol, 40 nmol, 200 nmol and 1000 nmol. For each synthesis scale there is a restriction regarding the length of the oligo.
- 10 nmol scale: 40 bases max. length
- 40 nmol scale: 150 bases max. length
- 200 nmol scale: 200 bases max. length
- 1000 nmol scale: 200 bases max. length
Guaranteed yield standard oligos
For non-labeled, non-HPLC/PAGE purified standard DNA oligos up to 30 bases we guarantee a minimum yield. However in many cases you will receive more than the guaranteed minimum!
- 10 nmol scale: 10 nmol min. yield
- 40 nmol scale: 20 nmol min. yield
- 200 nmol scale: 95 nmol min. yield
- 1000 nmol scale: 400 nmol min. yield
Biolegio has two synthesis protocols: 'High Purity' and 'B-pure'. The used protocol depends on the length of the oligo. This means that the protocols have been optimized for certain oligo lengths.
- High purity protocol is used for oligos between 1 and 79 bases.
- All DNA oligos longer than 80 bases are synthesized on our B-pure synthesis protocol.
Each protocol delivers the highest possible coupling efficiency for the specified range.
The final yield of an oligo depends on the sequence. Some more exceptional sequences with e.g. multiple consecutive dG's will result in a lower yield. As such the yields will be higher for by far most of the sequences.
Format & concentration
Your DNA oligo can be shipped dry or dissolved, annealed or mixed. Standard DNA oligos are delivered dissolved in a tube in a concentration of 100 µM (100 pmol/μl). Oligo's ordered dissolved on the 1000nmol synthesis scale will be delivered in a concentration of 1mM (1 nmol/µl).
- H2O buffer
Demineralized water with a resistance of ...Ohm
- LoTE buffer
TE buffer with a reduced concentration of EDTA
- TE buffer
The standard at Biolegio with a pH value of 8.0 for optimal stability
DNA oligos can be delivered in any format (plates, tubes, etc.), concentration or combination. Have a look at our oligo dispensing service for more information about this topic.
All DNA oligos are synthesized under low salt conditions. This avoids the need for purification for most basic molecular biology applications. Additional purification is available for more sensitive applications. Furthermore, we recommend purification for oligos longer than 40 bases.
We offer three different forms of purification:
- Standard desalting
Modifications can be incorporated either during (direct) or after (indirect) synthesis. You can choose from over 300 modifications. If your desired modification is not listed on our website, please contact us.
How can I add a modification to my oligo?
Select the modifications of your choice in our webshop or in our excel ordering sheet. In the webshop you can have instant insight in pricing.
How can I add mixed bases to my oligo?
Use the standard code letters for mixed bases / wobbles.
How can I order DNA/RNA hybrid oligos?
DNA/RNA hybrid oligos can be ordered in with some minor adjustments in the sequence. Mark the RNA bases by placing a lower case 'r' in front of them. For DNA bases place a lower case 'd' in front. For 'chemistry' choose DNA/RNA hybrid.
Storage and stability
Unmodified oligos can be used for at least 12 months when stored at -20°C. Use nuclease-free, sterile water for working solutions. Oligonucleotides modified with fluorescent dyes should be kept away from light as much as possible. Avoid repeated freeze-thaw cycles as this process can lead to degradation of the oligo.
All your DNA oligos are delivered with a specification sheet including GC-content, molecular weight, melting temperature (Tm), OD value, sequence and quantity in μgram and nmol.
A stringent quality control system ensures that you can expect the quality of our oligonucleotides to be of the highest standard. Before the synthesis, all chemicals are checked to meet our quality standards. During the fully automated oligo synthesis, all steps of the synthesis are monitored by multiple control functions on our DNA synthesizers. This way we can assure the coupling efficiency of the synthesis to meet our demands.
Synthesis is followed by further quality controls to guarantee the quality of the oligos. All oligos are routinely analyzed by optical density (OD) measurement. In addition, oligos are analyzed by gel electrophoresis and Liquid Chromatography Mass Spectrometry (LCMS). If oligos do not meet our requirement they are resynthesized. The result of our stringent QC process is a product of the highest standard. All oligos are delivered with our product specification sheet, which includes: % GC content, yield in nmol and μgram, OD, melting temperature and molecular weight (MW).