Overview

Synthetic sgRNA

 
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Synthego- Synthetic sgRNA CRISPR Kit

High-efficiency guide RNAs for knockouts and knock-ins.

More Efficient and Consistent Editing

Synthego's Synthetic single guide RNAs (sgRNAs) for genome engineering generate more efficient and consistent editing results than any other type of guide RNAs

Highest Indel Frequencies- up to 99%

Making it easy to efficiently conduct knockout or knock-ins in virtually any cell type.

Available with site-specific chemical modifications

Greater stability and decreased activation of the cellular innate immune response, resulting in superior editing in essentially every experiment.

Best Reproducibility

100% DNA Free - No risk of integrating foreign DNA into cell line- Extremely pure synthetic gRNA synthesis leads to unparalleled editing consistency

 

Synthetic sgRNA

 

Achieve up to 90% editing efficiency with the highest quality synthetic sgRNA in the market. Biolegio is the first company to deliver a production scale, full length 100-mer synthetic sgRNA product at a practical price and volume.

Unlike custom developed solutions that are expensive and can take weeks before transfection readiness, CRISPRevolution sgRNA ships up to 4X faster and 80% lower cost.

Synthego

CRISPRevolution

Synthetic RNA.

sgRNA KIT

sgRNA KIT

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01

Synthego

An optimized 80mer Synthego Scaffold is added on the 3′ end of your target sequence. For example, you will receive a sgRNA with the following format:

 

5′-AAUUUCACAGCUGCACAUA+Synthego Scaffold-3′

 

 

Synthego 100-mer sgRNA (MS Trace). Large single peak represents full-length product.

 

IVT-derived 105-mer guide RNA (MS Trace). Large n-1, n+1 and n+2 species are present.

 
02

CRISPRevolution

CRISPRevolution sgRNA Efficiency & Consistency

Hi Quality In = High Quality Out

The chart below compares the editing efficiency (solid bars) and consistency (black lines) of sgRNAs against in vitro transcribed guide RNAs in HEK293T cells. The experiment was conducted by a third-party using three different gene targets and was replicated three times.

 

 
03

Synthetic RNA.

Working with stem cells or other difficult targets?
Try Chemically Modified Synthetic RNA.

Chemically modified sequences can provide additional RNA stability with improvements in editing efficiency for particular cell types (e.g. stem cells, K562, prokaryotic) and genomic targets that prove otherwise challenging to edit. For a chemically modified version of our synthetic RNA, we offer 2’-O-methyl analogs and 3’ phosphorothioate internucleotide linkages at the first three 5’ and 3’ terminal RNA residues.

 
04

sgRNA KIT

  • Editing Efficiency ++++
  • Excellent Reproducibility
  • General Cell Types and Basic Applications
  • Full Length 100-mer Single Guide
  • Your 17-20nt Target Sequence
  • No Annealing
  • Material for 10-20 Transfections
  • Quantity: 3 nmol by OD260
  • TE Buffer
  • Nuclease-free Water
  • Ships in 5-7 Business Days
 
05

sgRNA KIT

*chemically modified

 

  • Editing Efficiency +++++
  • Excellent Reproducibility
  • Primary and Stem Cells; Therapeutic Applications
  • Full Length 100-mer Single Guide
  • Your 17-20nt Target Sequence
  • No Annealing
  • Material for 10-20 Transfections
  • Quantity: 3 nmol by OD260
  • TE Buffer
  • Nuclease-free Water
  • Ships in 7-10 Business Days 
 
06

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