AMBeR Sequencing Primers

Dramatically improve your Sanger Sequencing reads in the first 50 bases

 

Sequencing Primers with Advanced Mobility for Better Resolution, for when it's critical to read directly after the primer. 

Read from the very first Base

 

A common challenge of DNA sequencing with the Sanger method is typically the poor read quality in the first 50 bases of the sequence due to primer binding, the nature of capillary electrophoresis physics and the optimal range of polymers utilised to sort by size.

Utilising proprietary modifications within AMBeR primers adjust and improve the mobility of the smallest fragments immediately following the primer, leading to better resolution of the first 50 bases in your sequencing chromatograms (Figures 1 & 2).

No Special Considerations

 

No requirement to change you primer sequences or any other special considerations needed, just select AMBeR from the 5' Modification list when raising your order via our webshop and your primers will automatically be synthesised with our proprietary modifications.

 

 

Developed with Sanger Sequencing Experts

 

Developed in conjunction with NimaGen B.V. drawing on their expertise in developing consumables for Sanger Sequencing and CE Fragment Analysis, AMBeR Primer can be utilised in a full range of Sanger Sequencing applications and are compatible with any Dye Terminator Kits, Polymers and wider consumbles options and a full range of Genetic Analyzer Platforms

Compatible Platforms

Applied Biosystems® Genetic Analyzers:

• 3130
• 3130XL
• 3500
• 3500XL
• 3730
• 3730XL
• SeqStudio® (Flex)

Promega™:

• Spectrum Compact CE System

 

Applied Biosystems® and SeqStudio® are registered trademarks of ThermoFisher Scientific, Promega™ is a trademark of Promega Corporation